Org. Synth. 1941, 21, 74
[Isocaproic acid, α-amino]
Submitted by C. S. Marvel1
Checked by Homer Adkins and Robert Gander.
A. α-Bromoisocaproic acid. Five hundred grams (4.3 moles) of commercial isocaproic acid is mixed with 250 ml. of benzene in a 2-l. round-bottomed flask, and the water and benzene are removed by distillation through a short column until the temperature of the vapors reaches 100°. The temperature rises rapidly as soon as the last of the benzene is removed. The residual acid is cooled to room temperature, 743 g. (4.65 moles, 243 ml.) of dry bromine (Note 1) is added, and the flask is fitted with a long condenser and placed in an oil bath. The top of the condenser is connected to an empty 500-ml. Erlenmeyer flask which acts as a safety flask, and this in turn leads to a gas-absorption trap (Note 2). Ten milliliters of phosphorus trichloride is added to the mixture through the top of the condenser, and the flask is heated to 80–85°. The bromination proceeds smoothly at this temperature and is allowed to continue for 8–15 hours until the dark red color of bromine disappears from the condenser. When it has, the temperature is raised to 100–105° and kept there 2 hours. The contents of the flask are transferred to a 1-l. modified Claisen flask or a flask attached to a Widmer column and distilled. The fraction boiling at 125–131°/12 mm. is collected. The yield amounts to 530–550 g. (63–66%). The low-boiling fraction is mainly isocaproic acid (Note 3).
B. dl-Leucine. To 1.5 l. of technical ammonium hydroxide (sp. gr. 0.90) in a 3-l. round-bottomed flask is added 300 g. (1.56 moles) of α-bromoisocaproic acid. A rubber stopper is wired in, and the flask is allowed to stand for a week at room temperature. The crude leucine from four such flasks is collected on a filter and washed with 400 ml. of ethanol. This crop amounts to about 300 g. The ammonia is removed from the filtrate by heating the solution in a 12-l. flask on a steam cone overnight. The solution is concentrated under reduced pressure until vigorous bumping occurs (about 2.5 l.). The mixture is then cooled to about 15° and filtered. The precipitate is washed with 250 ml. of cold water and 250 ml. of 95% ethanol. The total yield of crude leucine in the two fractions is 440–460 g.
The amino acid is recrystallized by dissolving all the crude material in 12.5 l. of water heated to 95° on a steam cone. The hot solution is treated with 20 g. of Norit for 30 minutes and filtered hot. An equal volume of 95% ethanol is added immediately, and the flask is placed in the ice chest overnight. The crystalline material is collected on a filter and washed with 200 ml. of 95% ethanol. The yield of pure leucine in this fraction is 290–300 g. An additional crop is obtained by evaporating the mother liquors under reduced pressure until considerable solid separates (liquid volume about 1 l.), adding an equal volume of ethanol, and cooling. This crop is washed with 100 ml. of cold water and then with 200 ml. of ethanol; it amounts to 60–65 g. The total yield of pure leucine is 350–365 g. (43–45%). It decomposes at 290–292° (uncor.) in a sealed capillary (Note 4).
is dried by shaking with 500 ml. of C.P. concentrated sulfuric acid
The low-boiling fractions (105–115 g.
) may be combined with the next portion of acid to be brominated, or several such fractions may be collected and brominated together. If this last is done only two-thirds as much bromine
is used as in the original run.
The amino nitrogen content of leucine
prepared in this way checks with the theoretical value.
has been prepared by the hydrolysis of isobutylhydantoin
with barium hydroxide
by reduction and hydrolysis of α-oximinoisocaproate
by racemization of l-leucine
by the action of ammonia
and hydrogen cyanide
followed by hydrolysis;5
by the action of heat on isobutylmalonylazidic acid
followed by hydrolysis; 6
by the action of ammonia
on α-bromoisocaproic acid
by the condensation of isobutyl halides and sodio aminomalonic ester8
or sodio benzoylaminomalonic ester9
followed by hydrolysis; by the condensation of isobutyraldehyde
and hippuric acid
followed by reduction and hydrolysis,10
by reduction of α-ketoisocaproic acid phenylhydrazone
by the action of sodium hypobromite
on ethyl isobutylmalonamate
by the action of hexamethylene tetramine
on α-chloro and α-bromoisocaproic acids;13
and by hydrogenation and subsequent hydrolysis of methallyl acetamidomalonic ester.14
The method described above is essentially that of Fischer7
and is the cheapest and best procedure for the synthesis of large amounts of this amino acid.
Chemical Abstracts Nomenclature (Collective Index Number);
sulfuric acid (7664-93-9)
hydrogen cyanide (74-90-8)
hydrogen bromide (10035-10-6)
phosphorus trichloride (7719-12-2)
ammonium hydroxide (1336-21-6)
barium hydroxide (17194-00-2)
Hippuric acid (495-69-2)
hexamethylene tetramine (100-97-0)
α-Bromoisocaproic acid (49628-52-6)
Isocaproic acid, α-amino,
isocaproic acid (646-07-1)
α-ketoisocaproic acid phenylhydrazone
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