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Org. Synth. 2020, 97, 232-244
DOI: 10.15227/orgsyn.097.0232
Discussion Addendum for: Synthesis of Substituted Indazoles via [3 + 2] Cycloaddition of Benzyne and Diazo Compounds
Anton V. Dubrovskiy*§1 and Richard C. Larockǂ
Original Article: Org. Synth. 2010, 87, 95
Discussion
The use of benzyne annulation chemistry for the construction of N-containing heterocycles has seen significant growth in the literature in the last 15 years. One of the practical routes to these heterocycles begins with a [3 + 2] cycloaddition of benzyne (generated in situ from o-silylaryl triflates and fluoride ion) and diazo compounds. As reported by the Larock group,2 the initial adduct, 3H-indazole, may further rearrange to form 1H-indazole (it depends on the nature of the diazo compound and the substituents on the benzyne). Our 2010 Organic Syntheses report3 presents a large-scale synthesis (4.98 g) of a 1H-indazole derivative from ethyl diazoacetate. After this article, additional approaches to a family of indazoles (1H, 2H, and 3H) have been published, starting from o-silylaryl triflates and diazo compounds. This discussion addendum is intended to cover these reports.

Topics covered will be:

- Reaction of benzynes with monosubstituted diazo derivatives;

- Reaction of benzynes with disubstituted diazo derivatives;

- Reaction of benzynes with a-diazo phosphonates.

Reaction of Benzynes with Monosubstituted Diazo Derivatives

As the early reports by Yamamoto4,5 and Larock2 disclose, the reaction of benzynes (such as 1) with monosubstituted diazo compounds 2 always leads to 1H-indazoles 4, as a result of rearrangement of the initially formed 3H-indazole 3 to the 1H-indazole 4 during the course of the reaction (Scheme 1). The rearrangement is supposedly a 1,3-hydrogen shift, or a series of two 1,5-hydrogen shifts. The difference between Yamamoto's and Larock's reports is in that the former4,5 utilizes KF in THF at room temperature with an 18-crown-6 additive, while the latter2 utilizes TBAF in THF at -78 °C followed by warming up to room temperature. Furthermore, if an excess of benzyne is present, the free N-H indazole 4 is further converted to an N-arylated derivative (5, in the case of the unsubstituted benzyne); a closely related transformation has been reported as a standalone procedure in 2006.6 In both Yamamoto's and Larock's reports, the in situ formation of 5 has been achieved using 2 or more equiv of the benzyne precursor in the presence of CsF in CH3CN at room temperature. Both procedures taken together allow one to obtain 1H-indazoles (free and arylated) with R = ester, ketone, and Ph, in 56-97% yields, using an unsubstituted benzyne. Using both procedures, TMS-substituted diazomethane (2, R = TMS) afforded a desilylated product (4, R = H) in a lower yield (43-50%), with addition of CH3OH to the reaction mixture being necessary.
v97p0232-2.gif
Scheme 1. Initial syntheses of 1H-indazoles by Larock and Yamamoto
The scope of the overall transformation was expanded in 2015, when Ma reported the reaction of CF3CHN2 (7) with o-silylaryl triflates (Scheme 2).7 In this case, running the reaction in THF at room temperature in the presence of CsF and a common phase transfer catalyst, [Et3NBn]Cl (TEBAC), provided the highest yield, 81% using the unsubstituted benzyne precursor 6. Perhaps due to the relatively large ratio of the diazo compound to the benzyne precursor (4/1), formation of the over-arylated indazole (9) has not been observed.
v97p0232-3.gif
Scheme 2. Synthesis of 3-trifluoromethyl-1H-indazoles by Ma
Monosubstituted diazo derivatives successfully react with substituted benzyne precursors, as demonstrated in all three reports mentioned above (Larock,2 Yamamoto,4,5 and Ma7). In most cases the yields are somewhat
(10-20%, on average) lower than with the parent benzyne and a mixture of regioisomers is usually observed with unsymmetrical benzynes. It is noteworthy that there are some exceptions to this generalization, as presented in Scheme 3. As such, 3-methoxybenzyne (derived from 10) cleanly afforded products 9 and 15 as single regioisomers with both CF3CHN2 and ethyl diazoacetate, as did 4-methoxybenzyne (derived from o-silylaryl triflate 12) in its reaction with CF3CHN2. In both cases, the observed regioselectivity is analogous to the reported coupling of these benzynes with other 1,3-dipoles and nucleophiles.6,8 4,6-Di-tert-butyl-substituted aryne precursor 14 resulted in a single isomer as well, likely due to its steric hindrance. Garg reported a single regioisomeric indazole 17 in 65% yield, starting from 3-(triethylsilyl)benzyne (derived from o-silylaryl triflate 16), also presumably due to steric reasons.9 Finally, Danheiser in his 2014 study of a strained ynamide (accessible from 18), reports the clean formation of heterocycle 19 as a single regioisomer, upon coupling of the strained N-tosyl-3-azacyclohexyne with ethyl diazoacetate.10
v97p0232-4.gif
Scheme 3. Reaction of diazo compounds with substituted benzynes and an analogue
An interesting extension of the process shown in Scheme 1 was reported in 2012 by Liu and co-workers,11 who performed the cycloaddition of monosubstituted diazo ketones 20 with benzyne in the presence of AgOTf (Scheme 4). The silver ion effectively blocked the N-1 atom of the initially formed 1H-indazole (presumably forming complex 21), causing the extra benzyne intermediate (3 equiv were used in the process) to arylate N-2. As a result, 2-aryl-2H-indazoles 22 were formed (one entry's structure was confirmed by X-ray analysis). Optimal conditions (TBAF in CH2Cl2 at room temperature, in the presence of 5 mol% of AgOTf) allows one to obtain 2-phenyl-3-ketoindazoles with aryl (72-95%), heteroaryl (49-62%) and cinnamyl (44%) functionality. The use of a diazo ester (R = CO2CH2Ph) resulted in formation of 2-phenyl- and 1-phenyl- (inefficient Ag+ blockage presumably) indazoles in 4/1 to 5/1 ratios, with an increased selectivity at higher loadings of AgOTf catalyst.
v97p0232-5.gif
Scheme 4. Synthesis of 2H-indazoles by Liu
Shi and Larock envisioned that diazo coupling partners can be generated in situ from stable and readily available N-tosylhydrazones 23.12 Indeed, by reacting o-silylaryl triflates (such as 6) with 10 different N-tosylhydrazones in the presence of CsF and [Et3NBn]Cl (TEBAC) in THF at 70 °C, the desired 1H-indazoles 26 were produced in generally good yields, with only trace amounts of over-arylated products (Scheme 5). The reaction has been most successful with aryl-substituted N-Ts hydrazones (56-85% yields). Pyridine-3-carbaldehyde-derived hydrazone provided a 68% yield of the product, but thiophene-2-carbaldehyde-derived hydrazone gave only a 36% yield of the indazole, and pivalaldehyde N-tosylhydrazone resulted in only a 33% yield. It is noteworthy that N-Ts hydrazones derived from aliphatic aldehydes and alkenyl aldehydes did not prove effective in the transformation. While an alternative route (Scheme 5, path B) is not ruled out, a strong absorption at 2053 cm-1 in the IR and the overall outcome of the reaction with the unsymmetrical benzyne suggests that the major pathway in this process proceeds through formation of the intermediate diazo compound (path A).13
v97p0232-6.gif
Scheme 5. Synthesis of 1H-indazoles from N-tosylhydrazones by Shi and Larock
In 2012, Novák and co-workers employed a new imidazolylsulfonate-based benzyne precursor 27 in a coupling with several N-tosylhydrazones (28) under Shi and Larock's conditions (Scheme 6). The authors were able to successfully prepare a series of 1H-indazoles 29 (6 examples, 51-71% yields, EWG and EDG groups on the aryl ring are tolerated).14
v97p0232-7.gif
Scheme 6. Synthesis of 1H-indazoles using an imidazolylsulfonate benzyne precursor by Novák

Reaction of Benzynes with Disubstituted Diazo Derivatives

As our lab showed in 2008,2 stabilized disubstituted diazomethane derivatives 31 provide 3,3-disubstituted 3H-indazoles, [3 + 2] cycloaddition products 32, upon their coupling with o-silylaryl triflates 30 in the presence of CsF in CH3CN at room temperature (Scheme 7). For example, when one of the substituents is a Ph or CH2Ph group and another substituent is Ph or ester, 3,3-disubstituted 3H-indazoles 32 are produced in 44-87% yields. However, in many cases the carbonyl-containing functional group further undergoes an overall 1,3-migration, and N-substituted 1H-indazoles 33 are produced. The mechanism of the migration (32 to 33) is not clear, but is suggested to be a Fries-like ionization-recombination mechanism.15,2 In the case with R1 = Ph and R2 = CO2Et, the regular [3 + 2] product (32) was formed in a 72% yield, while the rearranged product (33) was formed in a 25% yield. Interestingly, in 2018, a change in conditions to TBAF/acetone at 0 °C allowed Peng and co-workers to isolate a similar 3H-indazole 32 (R1 = Ph and R2 = CO2Me) in an 85% yield, with no mention of any rearranged product being formed.16 In contrast, if this diazo derivative is generated in situ from the N-Ts hydrazone, exclusive migration of the ester group was observed, as reported by Shi, to form the corresponding 1H-indazole derivative 33 in a 63% yield.13
In the case of diazo substrates containing a ketone group, rearranged products 33 (N-keto 1H-indazoles) were the only products isolated using the Larock procedure.2 A ketone functional group seems to selectively migrate in the presence of an ester, amide, and aryl group. The resulting products were cleanly produced in 83-92% yields. If both substituents are ketone groups (acetyl) or both are ester groups (CO2Et), one of those groups migrates during the reaction, producing N-keto 1H-indazoles (33, R1 = ketone, 97% yield) or N-ester 1H-indazoles (33, R1 = ester, 85% yield). The use of Shi's procedure13 and ketone-containing N-Ts hydrazones as surrogates for diazo compounds resulted in complex mixtures under the reaction conditions.
v97p0232-8.gif
Scheme 7. Reaction of disubstituted diazomethanes with benzyne precursors
It is noteworthy that cyclic diazo compounds stabilized by two ketone or ester groups (34-36) have been recovered unreacted after the reaction under Larock's conditions2 (Scheme 8). Similarly, in 2017 Zhai and co-workers reported 34 to be unreactive and 37-39 to produce complex mixtures upon reaction with the benzyne precursor in the presence of TBAT (tetrabutylammonium triphenyldifluorosilicate) in CH3CN.17 Interestingly though, the structurally related cyclic diazo ketones 40 and 41 provided [3 + 2] cycloaddition products with benzyne in 93 and 79% yields respectively.
v97p0232-9.gif
Scheme 8. Cyclic diazo compounds explored in their coupling with benzyne
In their article, Zhai and co-workers reported the scope of this useful transformation and were able to successfully engage 8-substituted derivatives of 40 and 41 to produce the corresponding spiroindazoles in 66-95% yields.17 Some of these spiro-3H-indazoles (42) could further be rearranged to fused 2H-indazoles 43 via heat- (120 °C in toluene) or acid- (TFA, weakly acidic silica and CHCl3) mediated rearrangement (Scheme 9). Additionally, one spiro-3H-indazole (44) was reduced to a functionalized 1H-indazole 45 with NaBH4 in DCM/CH3OH (68% yield, structure confirmed by X-ray analysis).
v97p0232-10.gif
Scheme 9. Reactions spiroindazoles by Zhai
Similarly, 3-diazoindolin-2-ones (46) undergo a [3 + 2] coupling with benzyne (Scheme 10). In 2017, Reddy and co-workers were able to produce spirooxindoles 47 in 75-92% yields using CsF in CH 3CN at room temperature.18 The reaction tolerated methoxy, nitro, and halide substituents, and the N atom could be unprotected (N-H), as well as substituted (R = methyl, ethyl, propargyl, allyl, benzyl, and phenyl). Zhai and co-workers reported an alternative set of conditions, TBAT in THF at room temperature, which allowed them to produce spirooxindoles 47 in 80-98% yields with a similar transformation scope.19 Under the reaction conditions, N-H, N-Ac, and N-Ts substrates did not result in the desired product. Interestingly, Zhai and co-workers also reported isomerization of the spirooxindoles 47 produced into fused 2H-indazole derivatives 48 (indalozo[2,3-c]quinazolin-6(5H)-ones) under thermal conditions (heating at 120 °C in toluene). Eight derivatives 48 were successfully produced in 85-99% yields; one of the products was characterized by X-ray crystallography.
v97p0232-11.gif
Scheme 10. Synthesis of fused 2H-indazole derivatives by Zhai
Recently, Zhai and co-workers extended the scope of their original transformation (Scheme 10)20 to include N-PMB and N-MOM derivatives 46, but the authors were also able to isomerize spirooxindoles 47 into indalozo[2,3-c]quinazolin-6(5H-ones 48 upon milder reaction conditions (TFA in DCM at room temperature), rather than heating them at 120 °C. In fact, the two steps could be successfully combined into a one-pot procedure (93% yield overall, on a model substrate). They were also able to extend the reaction to eight examples of C=NTs and C=NMs analogues of 3-diazoindolin-2-ones (46) and produce the corresponding products in 49-70% yields. It is noteworthy that the resulting derivatives were quite stable under thermal or acidic conditions (i.e., did not rearrange to analogs of 48).

Reaction of Benzynes with α-Diazo Phosphonates

In 2008, Larock and co-workers disclosed that the reaction of triethyl diazophosphonoacetate (49) with excess benzyne precursor (2.4 equiv) conveniently affords an N-phenyl 1H-indazole 50 in a 55% yield (Scheme 11).2 It was not clear at the time at which stage the phosphonate group is lost, prior to or after the [3 + 2] cycloaddition.
In 2017, Ramana and co-workers investigated the reaction of analogs of the Ohira-Bestmann reagent (OBR) (51) with a benzyne precursor and several Michael acceptors in the presence of CsF in CH3CN (Scheme 11).21 OBR, widely known for its utility in alkyne synthesis,22 is known to undergo a base-mediated loss of the acyl group in the first step of its reactions. Authors have argued that by exchanging the traditional alkoxide base for a fluoride (which, on one hand, is not as basic but, on the other, has high affinity for a phosphorous atom) it should be possible to cause OBR to lose the phosphonate, and not the acyl group. Indeed, this seems to be the case. The coupling of analogs of OBR (51) with o-silylaryl triflates (30) in the presence of CsF in CH3CN has produced 1H-indazoles 52 in 68-85% yields, consistent with the earlier Larock findings.2 In all cases, to avoid over-arylation (at the N atom), excess of a Michael acceptor (4.4 equiv of methyl acrylate or acrylonitrile) has been used, thus producing N-alkylated versions of 1H-indazoles 52. Alkyl, aryl, and heteroaryl (indolyl) ketone analogs of OBR have reacted with similar efficiency. Using an ester rather than a ketone (51, R1 = OEt) significantly decreased the yield (31%) of this transformation. Mechanistic investigations conducted by Ramana suggest that loss of the phosphonate group from the OBR occurs within two hours under the reaction conditions (CsF, CH3CN, room temperature) and, while it does not prove that the group is lost exclusively before the [3 + 2] cycloaddition with the benzyne, it provides a plausible mechanistic explanation for the reaction's outcome.
v97p0232-12.gif
Scheme 11. Reaction of α-diazo phosphonates with benzynes
In 2018, Peng and co-authors disclosed that the reaction of dimethyl α-diazo phosphonates (53, R1 = CH3) with the benzyne precursor under a variety of fluoride-containing conditions consistently provided 1H-indazoles 54 (Scheme 12).23 In all cases, the phosphonate group was lost (consistent with Larock and Ramana) and, interestingly, no N-arylation products were observed. The optimal set of conditions, CsF in CH3CN at 40 °C, allowed the authors to obtain 1H-indazoles 54 with 3-aryl substitution (64-96% yields) and 3-alkyl substitution (82-92%). Note that these 3-alkyl-1H-indazoles are difficult to obtain; this procedure is one of the few examples among the papers reviewed in this addendum to accomplish that, along with the route to 3-CF3 indazoles reported by Ma.7
v97p0232-13.gif
Scheme 12. Synthesis of 1H-indazoles and 3H-indazole-3-phosphonates by Peng
Furthermore, Peng and co-workers envisioned that by modifying the phosphonate group, its loss (or migration followed by loss) could be suppressed. Indeed, by switching to diisopropyl α-diazo phosphonates (53, R1 = iPr, instead of the dimethyl-containing original substrates, the authors were able to suppress the migration and form diisopropyl 3-alkyl and aryl-3H -indazole-3-phosphonates 55 in 91-99% (for alkyl) and 75-96% (for aryl) yields. The optimal set of conditions was found to be TBAF in acetone at 0 °C. The reaction works reasonably well with a substituted electron-rich dioxolane-containing benzyne (88%) but is less efficient with the electron-deficient difluorobenzyne (47%, common trend in benzyne chemistry). Peng's method seems to be the first example of the synthesis of 3H-indazole-3-phosphonates, substrates that combine two medicinally interesting scaffolds, an indazole and a phosphonate.

Concluding Remarks

The field of benzyne-mediated transformations has continued its growth in the last decade. More and more procedures have appeared in the literature that allow the conversion of readily available starting materials into much more complex molecules, particularly, nitrogen-containing heterocycles. One can see from the discussion addendum how every reported finding increases the scope and efficiency of prior work and opens up new synthetic pathways to previously inaccessible structural frameworks. The ease of accessing these heterocyclic frameworks, many of which have established or potential bioactivity, will undoubtedly contribute to the future of medicinal chemistry.

References and Notes
  1. Department of Physical and Applied Sciences, University of Houston-Clear Lake, Houston, Texas 77058, United States. Email: dubrovskiy@uhcl.edu. ORCID: 0000-0001-5917-7394.
  2. Liu, Z.; Shi, F.; Martinez, P. D. G., Raminelli, C.; Larock, R. C. J. Org. Chem. 2008, 73, 219-226.
  3. Shi, F.; Larock, R. C. Org. Synth. 2010, 87, 95-103.
  4. Jin, T.; Yamamoto, Y. Angew. Chem. Int. Ed. 2007, 46, 3323-3325.
  5. Jin. T.; Yang, F.; Yamamoto, Y. Collect. Czech. Chem. Commun. 2009, 74, 957-972.
  6. Liu, Z.; Larock, R. C. J. Org. Chem. 2006, 71, 3198-3209.
  7. Sun, L.; Nie, J.; Zheng, Y.; Ma, J.-A. J. Fluorine Chem. 2015, 174, 88-94.
  8. (a) Shi, F.; Waldo, J. P.; Chen, Y.; Larock, R. C. Org. Lett. 2008, 10, 2409-2412. (b) Lu, C.; Dubrovskiy, A. V.; Larock, R. C. J. Org. Chem. 2012, 77, 2279-2284. (c) Spiteri, C.; Keeling, S.; Moses, J. E. Org. Lett. 2010, 12, 3368-3371.
  9. Bronner, S. M.; Mackey, J. L.; Houk, K. N.; Garg, N. K. J. Am. Chem. Soc. 2012, 134, 13966-13969.
  10. Tlais, S. F.; Danheiser, R. L. J. Am. Chem. Soc. 2014, 136, 15489-15492.
  11. Wang, C.-D.; Liu, R.-S. Org. Biomol. Chem. 2012, 10, 8948-8952.
  12. Li, P.; Zhao, J.; Wu, C.; Larock, R. C.; Shi, F. Org. Lett. 2011, 13, 3340-3343.
  13. Li, P.; Wu, C.; Zhao, J.; Rogness, D. C.; Shi, F. J. Org. Chem. 2012, 77, 3149-3158.
  14. Kovács, S.; Csincsi, Á. I.; Nagy, T. Z.; Boros, S.; Timári, G.; Novák, Z. Org. Lett. 2012, 14, 2022-2025.
  15. Yamazaki, T.; Baum, G.; Shechter, H. Tetrahedron Lett. 1974, 4421-4424.
  16. Chen. G.; Hu, M.; Peng, Y. J. Org. Chem. 2018, 83, 1591-1597.
  17. Cheng, B.; Bao, B.; Zu, B.; Duan, X.; Duan, S.; Li, Y.; Zhai, H. RSC Adv. 2018, 7, 54087-54090.
  18. Reddy, B. V. S.; Reddy, R. R. G.; Thummaluru, V. R.; Sridhar, B. ChemistrySelect 2017, 2, 4290-4293.
  19. Cheng, B.; Zu, B.; Bao, B.; Li, Y.; Wang, R.; Zhai, H. J. Org. Chem. 2017, 82, 8228-8233.
  20. Cheng, B.; Li, Y.; Zu, B.; Wang, T.; Wang, R.; Li, Y.; Zhai, H. Tetrahedron 2019, 75, 130775.
  21. Phatake, R. S.; Mullapudi, V.; Wakchaure, V. C.; Ramana, C. V. Org. Lett. 2017, 19, 372-375.
  22. (a) Ohira, S. Synth. Commun. 1989, 19, 561-564. (b) Mueller, S.; Liepold, B.; Roth, G.; J.; Bestmann, H. J. Synlett 1996, 1996, 521-522.
  23. Chen, G.; Hu, M.; Peng, Y. J. Org. Chem. 2018, 83, 1591-1597.

Anton V. Dubrovskiy received his Specialist (B.S./M.S.) degree from the Higher Chemical College in Moscow, Russia in 2007. He received his Ph.D. from Iowa State University under the guidance of Prof. Richard C. Larock in 2012. His research has focused on the development of aryne-mediated synthetic methodologies. Following postdoctoral work at the California Institute of Technology with Prof. Sarah Reisman in the area of total synthesis, Dr. Dubrovskiy joined the chemistry faculty of the University of Houston-Clear Lake in 2014, where he is currently an Assistant Professor of Chemistry.
Richard C. Larock is Distinguished Professor and University Professor Emeritus at Iowa State University where he taught from 1972 to 2011. He received his B. S. at the University of California at Davis in 1967 and his Ph.D. in 1972, under the direction of Prof. Herbert C. Brown. He then worked as an NSF Post-doctoral Fellow at Harvard University in Prof. E. J. Corey's group. Prof. Larock is a pioneer in the use of palladium catalysts in organic synthesis, particularly in the synthesis of carbocycles and heterocycles, and contributed also to the synthesis and characterization of biopolymers and biocomposites.